ASTM D3273 Mold Growth Resistance Testing for Coatings & Building Materials
Resistance to the growth of mold on the surface of interior coatings (paints and varnishes) in an environmental chamber is evaluated by ASTM D3273. This test mainly focuses on establishing the relative resistance of paint films to the propagation of mold and mildew under perfect fungal growth parameters.

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- Overview
- Scope, Applications, and Benefits
- Test Process
- Specifications
- Instrumentation
- Results and Deliverables
Overview
ASTM D3273 evaluates the resistance of interior coatings and materials to mold growth under high humidity conditions. The test exposes coated panels to a controlled environment that promotes fungal growth, allowing assessment of how effectively the coating inhibits mold development over time.
This method is essential for determining the durability and hygienic performance of coatings used in moisture-prone environments. It helps ensure that materials resist fungal contamination, maintain appearance, and provide long-term protection in areas with high humidity and poor ventilation.

Scope, Applications, and Benefits
Scope
ASTM D3273 defines a procedure for assessing the resistance of paint films and coatings to mold growth in a controlled environmental chamber. It provides a standardized rating system based on the extent of fungal coverage on the surface.
- Applicable to interior coatings and painted surfaces
- Evaluates resistance to fungal growth under high humidity
- Uses controlled temperature and humidity conditions
- Provides visual rating based on mold coverage
- Suitable for product development and quality control
- Helps compare antifungal performance of coatings
- Supports durability and hygiene assessment
Applications
- Interior wall and ceiling coatings
- Paints used in humid environments
- Building and construction materials
- Healthcare and clean environments
- Coating formulation development
Benefits
- Evaluates resistance to mold and fungal growth
- Helps improve indoor air quality and hygiene
- Supports development of antifungal coatings
- Ensures long-term coating durability
- Enables comparison between coating systems
Test Process
Specimen Preparation
Coated panels are prepared and conditioned before testing.
1Chamber Exposure
Specimens are placed in a controlled high-humidity environment promoting mold growth.
2Growth Observation
Surfaces are periodically inspected for fungal development.
3Rating Assignment
Mold growth is rated using a standardized visual scale.
4Technical Specifications
| Parameter | Details |
|---|---|
| Material Type | Coated panels and interior coatings |
| Environment | High humidity and warm temperature |
| Duration | Typically several weeks exposure |
| Measurement | Visual mold growth rating |
| Rating Scale | Numerical scale (0–10) |
| Output | Degree of fungal resistance |
| Chamber Type | Environmental growth chamber |
Instrumentation Used for Testing
- Environmental test chamber
- Humidity and temperature control system
- Sample panels and holders
- Visual inspection tools
- Documentation system
Results and Deliverables
- Mold growth rating (0–10 scale)
- Exposure conditions (temperature and humidity)
- Visual observations of fungal coverage
- Sample identification details
- Compliance statement with ASTM D3273
Frequently Asked Questions
ASTM D3273 is designed to evaluate how well coatings resist mold growth under high humidity conditions. It helps determine the effectiveness of coatings in preventing fungal contamination and maintaining surface integrity in moisture-prone environments.
Mold resistance is essential to maintain hygiene, prevent surface degradation, and avoid health risks. Coatings with poor resistance can develop visible fungal growth, leading to aesthetic damage and reduced durability over time.
High humidity creates ideal conditions for fungal growth. The controlled environment ensures consistent exposure, allowing accurate evaluation of coating resistance under worst-case conditions.
Temperature influences fungal growth rates, with warm conditions promoting faster development. Controlled temperature ensures consistent testing conditions.
It focuses specifically on coating surfaces and visual mold growth under controlled humidity, while other tests may evaluate microbial resistance using different methods.
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