Ultraviolet/Visible/Near Infrared Spectroscopy (UV/VIS/NIR) Testing

Ultraviolet/Visible/Near Infrared Spectroscopy (UV/VIS/NIR) Testing Services

The basic principle of Ultraviolet/Visible/Near Infrared Spectroscopy (UV/VIS/NIR) involves analytics techniques using a spectrophotometer to measure how much of a sample is absorbed in the UV/VIS/NIR range. The sample is illuminated with UV/VIS/NIR light, and the energy absorption of the sample is measured. This testing technique is used in many industries, such as pharmaceuticals, food, petroleum, and chemistry, to analyze the components of a sample and the structure of a compound. 

To conduct the UV/VIS/NIR spectroscopy test, the sample must first be prepared and placed into a sample cell. The sample cell is a clear container that holds the sample and allows light to pass through it. The sample cell is then placed inside the spectrophotometer, where the sample is illuminated with UV/VIS/NIR light. The spectrophotometer measures the amount of light the sample absorbs and produces a graph of the absorption spectrum. The absorption spectrum is then used to determine the concentration of components in the sample, identify the components of the sample, and analyze the structure of the compound.

Video 01: UV-Visible spectroscopy

Common Uses of UV/VIS/NIR Testing

• Identifying and quantifying components in a sample.
• Analyzing the structure of a compound.
• Measuring the concentration of components in a sample.

Advantages of UV/VIS/NIR Testing

• Can be used to analyze a wide range of samples. 
• It is very sensitive and can detect very small concentrations of components. 
• Provides detailed information about the sample.

Limitations of UV/VIS/NIR Testing

• Cannot be used to analyze solid samples. 
• Light sources used can be expensive.
• Cannot be used to measure the concentration of components in a sample at very low concentrations.

Industrial Application of UV/VIS/NIR Testing

• Food Industry: analyzing food components, such as proteins, carbohydrates, and fats. 
• Pharmaceutical Industry: analyzing drugs and other pharmaceuticals. 
• Petroleum Industry: analyzing oil and other petroleum products. 
• Chemistry Industry: analyzing organic and inorganic compounds.