ASTM D5988-18 Determining Aerobic Biodegradation of Plastic Materials in Soil

ASTM D5988-18 technique involves determining the degree and rate of aerobic biodegradation of plastic materials, includi ng formulation additives, in contact with soil under laboratory circumstances. The values are considered as a standard when expressed in SI units.... Read More

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    ASTM D5988-18 is intended to be used with any plastic material that does not restrict the growth of bacteria and fungus in the soil. This test technique evaluates the degree of aerobic biodegradation as soil is a valuable source of inoculum for testing the biodegradability of plastics in the environment since it contains so many different species.


    ASTM D5988-18 consists of the selection of plastic material for the determination of aerobic biodegradability, obtaining soil as a matrix and source of inoculum, exposing the plastic material to the soil, measuring the carbon dioxide evolved by the microorganisms as a function of time, and assessing the degree of biodegradability. The CO₂ production measured for a material, expressed as a fraction of the measured or calculated carbon content, is reported with respect to time, from which the degree of biodegradability is assessed. The level of biodegradation expressed in percent is determined by comparing the BOD with the theoretical oxygen demand (ThOD). In using this alternative approach, however, the influence of possible nitrification processes on the BOD must be considered.


    Proportion of biodegradation’s standard error Se is calculated, as follows:
    Se = SQRT [(S2 test /n1) + (S2 blank /n2)] ×100/Ci
    n1 and n2= number of replicate test and blank digesters respectfully
    S= standard deviation of gaseous carbon
    95 % confidence limits (CL) calculated by
    95 % CL = % biodegradation ± (t × Se)
    t = t-distribution value for 95 % probability with (n1 + n2 − 2) degrees of freedom
    thus n = 3 + 3 − 2 = 4.


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