Top 20 Nanotechnology Testing Methods

Written by Dr. Bhargav Raval | Updated: September 10, 2025

Top 20 Nanotechnology Testing Methods

Nanotechnology is the application of matter at the atomic, molecular, and supramolecular levels for industrial purposes. The top 20 nanotechnology testing methods are given below:

Scanning Electron Microscopy (SEM): This involves using a focused beam of electrons to create high-resolution images of nanostructures and measure their size and shape.
Transmission Electron Microscopy (TEM): This involves using a beam of electrons to transmit through a thin sample to create high-resolution images of nanostructures and measure their size and shape.
Atomic Force Microscopy (AFM): This involves scanning the surface of a sample with a sharp probe to measure its topography, size, and shape with nanoscale resolution.
X-ray Diffraction (XRD): This involves measuring the diffraction pattern of X-rays that are scattered by a sample to determine its crystal structure and identify its composition.
Raman Spectroscopy: This involves using laser light to excite the molecules in a sample and measuring the scattered light to determine its chemical composition and structure.
Fourier Transform Infrared Spectroscopy (FTIR): This involves measuring the infrared light absorbed by a sample to determine its chemical composition and structure.
Dynamic Light Scattering (DLS): This involves measuring the size distribution of nanoparticles in a sample by analyzing the scattered light caused by Brownian motion.
Differential Scanning Calorimetry (DSC): This involves measuring the heat flow and temperature changes in a sample to determine its thermal properties and behavior.

Video 01: Nanotechnology

Surface Plasmon Resonance (SPR): This involves measuring the changes in the refractive index caused by the binding of molecules to a nanoparticle surface to determine its binding affinity and kinetics.
Gel Electrophoresis: This involves separating and analyzing nanoparticles based on their size and charge using an electric field.
Zeta Potential Measurement: This involves measuring the electrokinetic potential of nanoparticles in a liquid medium to determine their surface charge and stability.
Magnetic Resonance Imaging (MRI): This involves using magnetic fields and radio waves to create high-resolution images of biological tissues and nanoparticles.
Small-angle X-ray Scattering (SAXS): This involves measuring the scattering pattern of X-rays by nanoparticles to determine their size, shape, and arrangement.

Ultraviolet-Visible Spectroscopy (UV-Vis): This involves measuring the absorbance or transmittance of light by a sample to determine its electronic structure and composition.
Inductively Coupled Plasma Mass Spectrometry (ICP-MS): This involves measuring the mass-to-charge ratios of ions generated from a sample by plasma to determine its elemental composition.
Field-Flow Fractionation (FFF): This involves separating and analyzing nanoparticles based on their size and shape using a flow field.
Dynamic Nuclear Polarization (DNP): This involves polarizing nuclear spins in a sample to enhance the sensitivity and resolution of nuclear magnetic resonance (NMR) spectroscopy.
Laser Light Scattering (LLS): This involves measuring the intensity and angular distribution of light scattered by a sample to determine its size and shape.
Electrochemical Impedance Spectroscopy (EIS): This involves measuring the electrical impedance of a sample to determine its electrochemical properties and surface interactions.
Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS): This involves measuring the mass-to-charge ratios of ions generated from a sample by a focused ion beam to determine its chemical composition and structure.