Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) is a laboratory testing technique used to analyze and identify small molecules in a sample. It is a powerful analytical tool that can detect and quantify trace levels of compounds in complex sample matrices. The technique combines two analytical systems, liquid chromatography (LC) and tandem mass spectrometry (MS/MS), to obtain detailed information about the sample.
Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) Testing Services
The basic principle of Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS) involves the separation of analytes using liquid chromatography (LC), followed by the identification and quantification of the analytes using tandem mass spectrometry (MS/MS). During the separation process, the sample is injected into the LC system and is then separated into individual components. The components are then introduced into the MS/MS system, where they are further fragmented and analyzed. The data obtained from the MS/MS system is then used to identify the components of the sample.
The results of LC-MS/MS are typically reported as concentrations of the detected compounds. This can be done in either absolute (ng/mL, ug/mL, etc.) or relative units (normalized to a standard compound). The data can also be reported as a graphical representation of the chromatogram, showing the retention time and peak area of the detected compounds.
Industries that use LC-MS/MS include pharmaceutical, environmental, food and beverage, and biomedical companies. The technique is used to detect, identify, and quantify trace levels of compounds in complex samples.
Common Uses of Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
- Identification and quantification of trace levels of compounds in complex samples.
- Detection of contaminants in pharmaceuticals, food, and environmental samples.
- Quantification of biomarkers in biological samples.
Advantages of Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
- It is a highly sensitive and selective technique.
- It can detect and quantify trace levels of compounds in complex samples.
- It can be used to identify a wide range of compounds.
Limitations of Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
- Time consuming.
- Requires specialized equipment and expertise.
- It is expensive.
Industrial Application of Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
- Pharmaceutical companies
- Environmental companies
- Food and beverage companies
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