Cryogenic Transmission Electron Microscopy (Cryo-TEM)
Cryo-TEM is a form of Transmission Electron Microscopy in which the polymer and nano-crystal samples are kept at cryogenic conditions, minimizing radiation damage and preventing water crystallization. It is used in structural biology for studying biomolecules in their hydrated state. Infinita Lab, USA enables our clients in the USA and worldwide to do the Cryo-TEM test at our network testing labs.
TRUSTED BY
Precision-driven testing for dimensional accuracy and compliance
- Overview
- Scope, Applications, and Benefits
- Test Process
- Specifications
- Instrumentation
- Results and Deliverables
Overview
Cryogenic Transmission Electron Microscopy (Cryo-TEM) is a highly advanced imaging technology used for viewing biological and soft matter samples at extremely low temperatures using an electron microscope.
In Cryo-TEM, samples are vitrified at extremely low temperatures using the process of rapid freezing, thereby eliminating the need for using chemicals for fixation and staining.
Using Cryo-TEM, researchers are able to view molecular structures, nanoparticles, viruses, and biomolecules at high resolution. Cryo-TEM is used extensively in the fields of biotechnology, structural biology, nanotechnology, and pharmaceutical research.
Scope, Applications, and Benefits
Scope
Cryo-TEM is used to examine the ultrastructure of biological specimens, nanomaterials, and soft matter by imaging samples preserved at cryogenic temperatures. The method provides high-resolution structural information while maintaining the original morphology of sensitive samples.
The scope includes:
High-resolution imaging of biomolecules and viruses
Structural analysis of proteins and macromolecules
Characterization of nanoparticles and nanomaterials
Imaging of soft materials and polymers
Study of cellular and molecular structures
Applications
Structural biology and protein research
Virus and biomolecule imaging
Nanotechnology and nanoparticle characterization
Materials science research
Benefits
Preserves samples in near-native hydrated state
Provides very high-resolution structural images
Suitable for delicate biological specimens
Minimizes sample damage during preparation
Enables detailed molecular and nanoscale analysis
Test Process
Sample Vitrification
The sample is rapidly frozen using liquid ethane or nitrogen to form a vitrified (glass-like) structure that preserves its natural state.
1Cryogenic Transfer
The frozen sample is transferred to the microscope under cryogenic conditions to prevent ice crystals
2Electron Imaging
A beam of electrons passes through the sample to produce high-resolution images of the internal structure.
3Image Processing
The captured images are processed and analyzed to study molecular structures and nanoscale features.
4Technical Specifications
| Parameter | Details |
|---|---|
| Technique Type | Electron microscopy imaging |
| Operating Temperature | Cryogenic temperatures (≈ −180°C) |
| Resolution | Near atomic-scale resolution |
| Sample State | Rapidly frozen vitrified samples |
| Applicable Materials | Biomolecules, viruses, nanoparticles, polymers |
| Imaging Method | Transmission electron imaging |
| Output Data | High-resolution micrographs and structural models |
Instrumentation Used for Testing
Cryogenic Transmission Electron Microscope
Cryo Sample Holder
Electron Beam Source
Vitrification System (Plunge Freezer)
Image Processing Software
Results and Deliverables
High-resolution Cryo-TEM images
Structural analysis of biomolecules or nanoparticles
Morphology and size distribution data
Molecular or nanoscale structure evaluation
Detailed microscopy analysis report
Why Choose Infinita Lab for Cryogenic Transmission Electron Microscopy?
At the core of this breadth is our network of 2,000+ accredited labs in the USA, offering access to over 10,000 test types. From advanced metrology (SEM, TEM, RBS, XPS) to mechanical, dielectric, environmental, and standardized ASTM/ISO testing, we give clients unmatched flexibility, specialization, and scale. You’re not limited by geography, facility, or methodology—Infinita connects you to the right testing, every time.
Looking for a trusted partner to achieve your research goals? Schedule a meeting with us, send us a request, or call us at (888) 878-3090 to learn more about our services and how we can support you. Request a Quote
Frequently Asked Questions
Cryo-TEM is an electron microscopy technique used to image biological and soft materials at extremely low temperatures, allowing researchers to observe structures in their natural hydrated state with very high resolution.
Samples are rapidly frozen to preserve their natural structure and prevent damage or structural changes that may occur during conventional preparation methods.
Unlike conventional TEM, Cryo-TEM preserves samples in a frozen hydrated state, reducing structural damage and providing more realistic images of biological or soft materials.
Cryo-TEM allows scientists to visualize the detailed structure of proteins, viruses, and biomolecules, helping researchers understand biological mechanisms and develop new drugs.
Cryo-TEM is generally considered minimally destructive because the sample is frozen and examined without significant chemical alteration.
Request a Quote
Submit your material details and receive testing procedures, pricing, and turnaround time within 24 hours.
Quick Turnaround and Hasslefree process
Confidentiality Guarantee
Free, No-obligation Consultation
100% Customer Satisfaction
