Liquid Chromatography-Mass Spectroscopy (LC-MS) is an analytical technique used for the separation, identification, andquantification of the chemical constituents of a sample. Testing labs under the Infinita Lab network provide the best LC-MS analysis for our clients based in the USA and other countries. ... Read More
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Liquid Chromatography-Mass Spectroscopy
Liquid Chromatography-Mass Spectroscopy (LC-MS) is an analytical technique that allows the separation, identification, and quantification of the sample constituents. It is the preferred separation technique for larger and non-volatile molecules like proteins and peptides. It’s also ideal for the separation of isomers (same mass) which cannot be differentiated in a mass spectrometer. With its high sensitivity, high detection selectivity, and multicomponent analysis capabilities, LC-MS is used for qualitative and quantitative analysis of trace elements, contaminants, additives, etc. in a wide range of industries from drug delivery, clinical research, biopharmaceuticals to textiles and environmental sciences.
In LC, a sample is injected and adsorbed on the stationary phase (porous medium) as the liquid mobile phase passes through the column to separate the compounds. Compounds are separated based on their weight and affinity towards the stationary and mobile phases. The separated analytes then pass through a mass detector that differentiates compounds by their mass-to-charge ratio (m/z). MS provides information about the identity, quantity, molecular weight, and purity of a sample.
We at Infinita Lab are committed to providing the best routine and advanced Liquid Chromatography-Mass Spectroscopy analyses for our clients based in the USA and across the world. Our testing laboratory has various state-of-the-art instruments and methodologies to perform LC-MS experiments.
Liquid Chromatography-Mass Spectroscopy (LC-MS) Common Uses
Analysis and quantification of drugs and metabolites contained in biological samples (pharmacokinetic studies)
Used in drug development methods such as in vivo drug screening, peptide, and glycoprotein mapping, etc.
Biochemical screening for genetic disorders such as tyrosinemia type 1, urine diseases, and other clinical research and testing.
Reliable measurements in the areas of steroid biochemistry
Analysis of natural products and the profiling of secondary metabolites in plants
Analysis of hydrocarbons and contaminants in diesel engine exhausts
Determination of chemical contaminants (such as aflatoxins) in complex food matrices
Identification and characterization of reaction intermediates and by-products
Qualitative and quantitative analysis of polymer extractable/leachable such as plasticizers, stabilizers, antioxidants, etc.
Advantages of Liquid Chromatography-Mass Spectroscopy (LC-MS)
Fast LC analysis times and reduced sample preparation that allows high throughput sample analysis
Able to perform simultaneous multianalyte analysis within a single run and minimal incremental cost
Excellent reproducibility of the results with the use of isotopically labeled internal standards (MS/MS)
Polarity switching for both positive and negative ions in a single analysis
High sensitivity – able to detect some compounds in the parts-per-billion (ppm) and -trillion (ppt) ranges (MS/MS)
Limitations of Liquid Chromatography-Mass Spectroscopy (LC-MS)
LC systems are expensive to maintain and need extensive training to operate when compared to GC
Stringent vacuum requirement
Mass spectrometers that couple to LC systems are expensive
The environmental conditions in the laboratory need to be well controlled to ensure system stability
The ionization techniques compatible with LC-MS produce very little fragmentation, which is useful for determining its molecular weight but does not provide any structural information of the molecules
Compounds must be soluble in common solvents such as water, alcohols, and acetonitrile
Compounds must be ionizable (usually polar)
Matrix effects (or ion suppression) occur because some components of the sample disturb the ionization mechanism, especially biological samples
Industries where Liquid Chromatography-Mass Spectroscopy (LC-MS) is used
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Select Other Metrology Testing Services From Catalogue
You can get your materials analyzed right here at Infinita Lab. Our network of material testing labs provides identification, quantitation, and mass analysis of materials using liquid chromatography-mass spectrometry (LC-MS).
Liquid chromatography-mass spectrometry (LC-MS) is used for quantitative and qualitative analysis of materials. It can detect trace levels of polymer extractables and leachables, plasticizers, surfactants, pharmaceuticals, etc. Higher sensitivity and specificity in detection can be achieved with the use of quadrupole spectrometers in LC-MS analysis.
Both techniques work on the same principle. However, during the separation process, gas chromatography-mass spectrometry (GC-MS) uses gaseous phase eluent to separate analytes, whereas liquid chromatography-mass spectrometry (LC-MS) uses a liquid solvent. Although LC-MS cannot handle gaseous or volatile samples, it can analyze thermally unstable samples like biological fluids.
ASTM E572 test method covers the analysis of stainless and alloy steels by Wavelength Dispersive X-ray Fluorescence Spectrometry (WDXRF). It provides rapid, multi-element determinations with sufficient accuracy to assure product quality.
The ASTM D2674 test is a standard test method for the analysis of sulfochromate etch solutions used in the surface preparation of aluminum. The ASTM D2674 standard specifies a method for determining the efficacy of an etchant used to prepare the surface of aluminum alloys for subsequent adhesive bonding.
An immunological method for quantization of Hevea Natural Rubber (HNRL) proteins using rabbit anti-HNRL serum. Rabbits immunized with HNRL proteins react to the majority of the proteins present, and their sera have the capability to detect most if not all the proteins in HNRL.
ASTM G65 measures the resistance of metallic materials to abrasion using the dry sand/rubber wheel apparatus. The quality, durability, and toughness of the sample are determined using this test. Metallic materials are ranked in their resistance to scratching abrasion under a controlled environment.
ASTM E2141 test methods provide accelerated aging and monitoring of the performance of time-dependent electrochromic devices (ECD) integrated in insulating glass units (IGU). This test helps to understand the relative serviceability of electrochromic glazings applied on ECD.
ASTM C724 test method is used in analyzing the quality and ease of maintenance of a ceramic decoration on architectural-type glass. This test method is useful in the acknowledgment of technical standards.
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