ASTM D5712: Elastomer Aqueous Extractable Protein Testing Services
ASTM D5712 test method is used to determine the amount of aqueous extractable protein present in Latex, Natural Rubber, and Elastomeric products using the Modified Lowry Method.
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- Overview
- Scope, Applications, and Benefits
- Test Process
- Specifications
- Instrumentation
- Results and Deliverables
What Is ASTM D5712 Elastomer Aqueous Extractable Protein?
ASTM D5712 is used to determine the amount of aqueous-extractable protein in natural rubber and latex-based elastomers. The test measures water-soluble proteins that can leach out from elastomeric products, which is critical for evaluating potential allergenic risks.
This method is widely used for medical and consumer products such as gloves and elastomeric components. It helps ensure product safety, supports regulatory compliance, and evaluates the effectiveness of manufacturing and washing processes in reducing protein content.
Applications and Benefits of ASTM D5712 Elastomer Aqueous Extractable Protein Testing
Scope
ASTM D5712 evaluates the concentration of extractable proteins in elastomers by extracting soluble proteins into an aqueous medium and quantifying them using standardized analytical techniques.
The test evaluates:
- Water-extractable protein content in elastomers
- Potential allergenic substances in rubber materials
- Effectiveness of post-processing treatments
- Consistency of production processes
- Suitability for medical and skin-contact applications
Applications
- Natural rubber latex gloves
- Medical and surgical devices
- Elastomeric seals, tubing, and components
- Consumer rubber products
- Healthcare and pharmaceutical materials
- Quality control in rubber manufacturing
Benefits
- Provides a standardized measurement of protein content
- Helps reduce allergenic risks in rubber products
- Supports compliance with safety standards
- Enables comparison between different materials
- Assists in improving manufacturing processes
Test Our ASTM D5712 Testing Procedure
Sample Preparation
Elastomer samples are cut into specified sizes and cleaned prior to testing.
1Protein Extraction
Samples are immersed in an aqueous solution to extract soluble proteins.
2Protein Quantification
Extracted proteins are measured using colorimetric methods such as modified Lowry technique.
3Data Recording & Evaluation
Protein concentration is calculated and evaluated against specified requirements.
4ASTM D5712 Test Parameters and Requirements
| Parameter | Details |
|---|---|
| Applicable Materials | Natural rubber latex and elastomers |
| Extraction Medium | Deionized water or phosphate buffer |
| Extraction Temperature | 23°C ± 2°C or up to 37°C |
| Extraction Time | 2 hours ± 10 minutes |
| Detection Method | Modified Lowry method |
| Measured Outputs | Protein content (µg/g or µg/dm²) |
- Water bath or incubator
- UV-Vis spectrophotometer
- Analytical balance
- Pipettes and volumetric glassware
- Sample preparation tools
- Data acquisition and analysis system
Equipment and Instrumentation Used for ASTM D5712 Testing
What You Receive: Test Report, Data, and Certification
- Extractable protein concentration values
- Allergenic risk assessment data
- Process efficiency evaluation
- Comparative material analysis
- Test condition summary
- ASTM compliance report
ASTM D5712 Elastomer Aqueous Extractable Protein FAQs
ASTM D5712 includes extraction temperature, extraction time, aqueous medium composition, sample mass, and protein detection method, determining levels of extractable proteins in elastomer materials under controlled laboratory conditions.
ASTM D5712 typically uses colorimetric or biochemical assays, such as modified Lowry method, to quantify protein concentration extracted from elastomer samples.
ASTM D5712 is commonly applied to natural rubber latex and elastomer products such as gloves, medical devices, and consumer products where protein content must be controlled.
ASTM D5712 reports extractable protein content typically in micrograms per gram, indicating potential allergenic risk and material cleanliness.
ASTM D5712 results may be influenced by extraction efficiency, assay sensitivity, and sample variability, requiring careful control of test conditions to ensure accurate protein quantification.
Why Choose Infinita Lab for Elastomer Aqueous Extractable Protein Testing?
At the core of this breadth is our network of 2,000+ accredited laboratories across the USA, offering access to over 10,000 testing methods and analytical services. From advanced materials characterization (SEM, TEM, RBS, XPS) to mechanical, chemical, environmental, biological, and standardized ASTM/ISO-compliant testing, we deliver unmatched flexibility, specialization, and scale. You are never limited by geography, facility, or methodology — Infinita Lab connects you to the right expertise and testing solution, every time.
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