Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials ASTM E2180. This t est is useful in determining the antimicrobial activity of hydrophobic antimicrobial-treated materials.... Read More
Average 30% Cost Savings
100% Confidentiality Guarantee
Free, No-obligation Consultation
100% Customer Satisfaction
TRUSTED BY ENGINEERS FROM
Antimicrobial Efficacy Testing ASTM E2180
Determining the Activity of Incorporated Antimicrobial Agent(s) In Polymeric or Hydrophobic Materials ASTM E2180. This test is useful in determining the antimicrobial activity of hydrophobic antimicrobial-treated materials.
Scope:
ASTM E2180 test standard is used to quantitatively test the antimicrobial effectiveness of incorporated agents in flat hydrophobic or polymeric materials. Other microorganisms like yeast and fungal conidia can be also determined using this test. To determine the antimicrobial activity, proper contact of microbial to the surface of antimicrobial is essential. It is difficult to spread the micro-organisms on hydrophobic surfaces like polymers. The ASTM E2180 test overcomes this obstacle by incorporating the test inoculum into an agar slurry that provides good contact of microorganisms with antimicrobial-treated hydrophobic surfaces.
Test Procedure:
A thin layer of the microorganisms inoculated agar slurry is used for the ASTM E2180 test. This agar slurry provides full contact between the polymeric or hydrophobic surfaces and the bacterial cultures. Then, the inoculated agar slurry is spread onto the test samples, and minimum triplicate samples are used for the untreated control material. The designated incubation time period is 24 hours. Then, surviving micro-organisms are recovered by eluting the inoculated agar slurry via the test substrate into neutralizing broth. After that, dilution series are made and then plated, followed by an incubation period of 48 hours at a specified temperature. Then, the agar plates are removed from the incubator, and bacterial colonies from each dilution series are computed and noted. To calculate the percent reduction of bacteria, a comparison of the treated vs. untreated samples is made. In some cases, the exposure periods might be increased to 96 hours if there are more resistant micro-organisms. Usually, Pseudomonas aeruginosa or Klebsiella pneumoniae and Staphylococcus aureus are used for the test. Other microorganisms such as yeast or fungi can also be used for the test using the same procedure.
Sample size:
The samples are 8 10 inches pieces. The squared samples of 3.03.0 inches are for the treated and control test materials. An untreated control sample is also necessary for the test.
Data:
The results of the test are expressed by the relation;
For log expression: geometric mean = (Log R1 +Log R2+Log R3)/3
where;
R = number of microorganisms recovered from the incubation period untreated or incubation period treated samples.
The percent expression is given by;
R(%)=Nu-Na Nu 100
where;
Nu= the antilog of the geometric mean of micro-organisms recovered from each incubation period (untreated samples) Na= the antilog of the geometric mean of micro-organisms recovered from each incubation period (treated samples)
ASTM E572 test method covers the analysis of stainless and alloy steels by Wavelength Dispersive X-ray Fluorescence Spectrometry (WDXRF). It provides rapid, multi-element determinations with sufficient accuracy to assure product quality.
The ASTM D2674 test is a standard test method for the analysis of sulfochromate etch solutions used in the surface preparation of aluminum. The ASTM D2674 standard specifies a method for determining the efficacy of an etchant used to prepare the surface of aluminum alloys for subsequent adhesive bonding.
An immunological method for quantization of Hevea Natural Rubber (HNRL) proteins using rabbit anti-HNRL serum. Rabbits immunized with HNRL proteins react to the majority of the proteins present, and their sera have the capability to detect most if not all the proteins in HNRL.
ASTM G65 measures the resistance of metallic materials to abrasion using the dry sand/rubber wheel apparatus. The quality, durability, and toughness of the sample are determined using this test. Metallic materials are ranked in their resistance to scratching abrasion under a controlled environment.
ASTM E2141 test methods provide accelerated aging and monitoring of the performance of time-dependent electrochromic devices (ECD) integrated in insulating glass units (IGU). This test helps to understand the relative serviceability of electrochromic glazings applied on ECD.
ASTM C724 test method is used in analyzing the quality and ease of maintenance of a ceramic decoration on architectural-type glass. This test method is useful in the acknowledgment of technical standards.
Send us a request
Process for testing
STEP 01
You share material and testing requirements with us
STEP 02
You ship your sample to us or arrange for us to pick it up.
STEP 03
We deliver the test report to your email.
Just share your testing requirements and leave the rest on us!
Free, no-obligation consultation
Guaranteed confidentiality
Quick turnaround time
Hassle-free process
Let us combine our capabilities to achieve success!!