Gas Chromatography (GC) Analysis Services

Gas Chromatography (GC) is a separation technique used to detect chemical components in a mixture. This method is widelyused in the separation, identification, and quantification of sample mixtures primarily based on volatility. ... Read More

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    GC Analysis Working Principle: 

    Chromatography is a technique that separates components in a mixture due to differences in partitioning behavior between mobile and stationary phases. Gas chromatography (GC) is one of the common chromatographic techniques for separating volatile compounds or substances. The mobile phase is a gas, such as helium, and the stationary phase is a high-boiling liquid adsorbed on a solid.

    First, the sample is introduced into an inert gas or carrier stream, usually helium or argon. Liquid samples must be vaporized before injection into the carrier. Sample components move through a packed column at a rate that is influenced by the degree of interaction of each component with the fixed non-volatile phase. Substances that interact more strongly with the stationary phase are retarded and separated from those that interact less. Once the components elute from the column, they can be quantified and/or collected by a detector for further analysis.

    Common uses of Gas Chromatography Analysis

    • Determination of purity of a particular substance.
    • Separating the different components of a mixture.
    • Routine analysis.
    • Research or analyze the compounds in different samples.

    Advantages of Gas Chromatography Analysis

    • The efficiency of GC allows the components of complex mixtures to be separated in a reasonable amount of time.
    • Gas chromatography is highly selective and can be used to analyze azeotropes and samples with similar boiling points.
    • Free control of operating parameters such as temperature programming, carrier gas flow, and split ratio adjustment helps resolve nearby peaks.
    • Gas chromatography is simple, automated, and offers rapid data analysis with relatively high accuracy, precision, and reproducible results.
    • Operating parameters such as flow rate, temperature and pressure can also be easily changed during a chromatographic run.

    Limitations of Gas Chromatography Analysis

    • Gas chromatography can only analyze helium/hydrogen volatile compounds with a molecular weight of around 1250u. Thermally labile compounds may be degraded by the high temperatures of the GC.

    Industrial applications of Gas Chromatography Analysis

    • Pharmaceuticals
    • Chemical industries
    • Petroleum industries

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