Types of Spectrophotometers 

The five main types of spectrophotometers are UV-Visible spectrophotometer, Infrared spectrophotometer, Fluorimeter, Raman spectrophotometer, and Nuclear Magnetic Resonance spectrophotometer. Each type of spectrophotometer is designed to measure the absorption or emission of light in a specific region of the electromagnetic spectrum and is used in a variety of fields such as biochemistry, chemistry, environmental science, materials science, and pharmaceuticals.

Last Updated: September 18th, 2023 First Published :

  

Types of Spectrophotometers 

The instrument used for measuring the intensity of light as a function of its wavelength is called a spectrophotometer. There are various types of spectrophotometers available in the market, each with its own unique features and capabilities. 

UV-Visible Spectrophotometer:

A UV-Visible measures the absorption of light in the ultraviolet (UV) and visible regions of the electromagnetic spectrum. This type of spectrophotometer is widely used in the fields of biochemistry, pharmacology, and environmental science to determine the concentration of a substance in a solution.

Infrared Spectrophotometer:

An infrared (IR) spectrophotometer measures the absorption of light in the infrared region of the electromagnetic spectrum. This type of spectrophotometer is used to identify and quantify the functional groups in a molecule. IR spectrophotometry is commonly used in the fields of organic chemistry, pharmaceuticals, and biochemistry.

Fluorimeter:

A fluorimeter is a type of spectrophotometer that measures the fluorescence of a sample. Fluorescence is the emission of light that occurs when a molecule absorbs light of a certain wavelength and then re-emits it at a longer wavelength. Fluorimeters are used in a variety of applications, including biological imaging, drug discovery, and environmental analysis.

Raman Spectrophotometer:

A Raman spectrophotometer measures the Raman scattering of light, which is the inelastic scattering of light by a molecule. This type of spectrophotometer is used to identify and quantify the chemical composition of a sample. Raman spectrophotometry is widely used in the fields of chemistry, materials science, and pharmaceuticals.

Nuclear Magnetic Resonance Spectrophotometer:

A Nuclear Magnetic Resonance (NMR) spectrophotometer is a type of spectrophotometer that measures the magnetic properties of atomic nuclei. This type of spectrophotometer is used to determine the structure and composition of molecules in a sample. NMR spectrophotometry is commonly used in the fields of chemistry, biochemistry, and pharmaceuticals.

The choice of spectrophotometer depends on the specific needs of the user, the type of sample being analysed, and the desired outcome.

Video 01:Spectrophotometer and its types

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