ASTM E394 Standard Test Method for Determination of Iron in Trace Quantities Using the 1,10-Phenanthroline Method 

ASTM E394 test method is used to determine the amount of iron in a sample ranging from 1 to 100 µg. This test method is designed to be used in the latter stages of iron determination. The performance of specimens evaluated using this method is expressed in SI units.... Read More

  • Average 30% Cost

  • 100% Confidentiality

  • Free, No-obligation

  • 100% Customer



    ASTM E394 is a standard for the determination of iron in trace quantities using the 1,10-phenanthroline test method. This test method can be used to determine trace levels of iron in a wide range of products, as long as the iron and sample matrix have been properly prepared and made soluble in water or another acceptable solvent. The amount of color created in this procedure is assumed to be proportionate to the amount of iron in the test solution and the calibration curve is linear over the spectral range.

    Test Procedure:

    The 1,10-phenanthroline combination with iron (II) ion is measured photometrically in the ASTM E394 test technique. With the addition of hydroxylamine hydrochloride, the sample is dissolved in a suitable solvent and the iron is reduced to a divalent state. After that, 1,10-phenanthroline is added to bring out the hue. The absorbance of the solution is measured using an appropriate photometer at approximately 510 nm after a brief reaction period. The solution’s absorbance remains steady for at least several months after the color has developed.

    Video 01: Measurement of Iron in Water by Phenanthroline Method

    Specimen Size: 

    The ASTM E394 technique uses samples with a pH of less than 2 in their solutions.


    As per the ASTM E394, the iron content of the sample is calculated as follows:

    Iron µg/g (ppm) = B/W


    B = micrograms of iron found

    W = grams of sample

    The iron content is reported to the nearest 0.01 µg/g(ppm).


    ASTM E394 covers the determination of iron in trace quantities using the 1,10-phenanthroline method. This test method can be used to determine trace levels of iron in a wide range of products.


    Core Materials Testing Services


    A variety of microscopy and spectroscopy tools available for precise measurements from the nano to the meter scale.

    Metrology Testing Services POPULAR TESTS:

    TEM SIMS Profilometry

    Materials Testing

    ASTM and ISO standard and custom chemical, mechanical, thermal, corrosion tests, etc. for all materials metals, ceramic or polymers.

    Materials Testing Services POPULAR TESTS:

    Nondestructive Testing Electrical Testing Chemistry Analysis

    Product Testing

    Thousands of tests for product quality and reliability under heat, humidity, temperature shock, vibration, drop, electrostatic discharge.

    Product Testing Services POPULAR TESTS:

    Vibration Testing Humidity Testing Accelerated Aging

    Looking for Material Testing?

    We have already delivered 10000+ Material Test results to top companies

      Send us a request

        Process for testing
          STEP 01

          You share material and testing requirements with us

          STEP 02

          You ship your sample to us or arrange for us to pick it up.

          STEP 03

          We deliver the test report to your email.

          Let’s work together!

          Share your testing requirements with us and we will be happy to assist you.

          ddd Just share your testing requirements and leave the rest on us!
          • Free, no-obligation consultation
          • Guaranteed confidentiality
          • Quick turnaround time
          • Hassle-free process


            Let us combine our capabilities to achieve success!!

              idea icon
              Want to connect with an expert before you leave?